Project title: Comprehensive, multimodal profiling of chromatin states and gene expression in single cells.

The main aim of this project is to develop an experimental protocol to simultaneously measure dozens of histone modifications and DNA-bound proteins, accessibility of the chromatin, and RNA molecules that are polyadenylated (protein coding genes) and non-polyadenylated (regulatory RNAs). This comprehensive approach will allow us to study single-cell genome-wide features at unprecedented resolution. To analyze big data generated by the new profiling protocol, we will develop a novel computational tool that would allow us to handle the multimodal data in a highly parallel manner. We will apply the multimodal profiling protocol to study the transcriptional dynamics of primary immune cells in response to stimulation with a bacterial endotoxin.

The total funding of the project is 3 786 270 PLN.