Ophthalmic Biology Laboratory

Principal Investigator: Andrzej Foik, PhD hab.

Most common vision disorders in humans have a genetic origin or develop secondarily as a result of other systemic diseases; e.g., diabetes, hypertension, arteriosclerosis, and others. Their prevalence increases and symptoms worsen with aging. Many visual pathway diseases are related to the degeneration of specific types of neuronal cells in the retina, while other neurons are preserved. Independently of their cause, the affected visual system develops pathological changes in the processing of visual information. At the more advanced stages of disease, progressive loss of neuronal cells at a particular level of the visual pathway results in loss of excitation of the neurons at subsequent levels. The decrease in activity of a neuronal cell leads to its degeneration. This mechanism is responsible for secondary loss of healthy neuronal cells, not directly affected by the disease, at later stages of disease progression. These processes develop gradually, in most cases throughout many years, providing a chance to prevent the secondary pathological changes.

The ultimate goal of the OBi Group is to prevent loss of neuronal cells, restraining pathological functional plasticity, and to restore visual signal processing in the diseased retina by adjusting and strengthening the activity of the remaining neuronal cells.

Our molecular biology lab utilizes the latest advancements in genetics and viral tracing techniques to discover new viral gene therapies to support cellular structures that are responsible for the detection of visual signals.

Using in vivo and in vitro techniques in the electrophysiological lab, we analyze local and distant communication between the different populations of neurons responsible for visual information processing in healthy and diseased retina and brain. This analysis enables us to gain insight into critical changes in the functioning of the neuronal networks of vision in pathological conditions. Electrophysiology is also a valuable tool for testing the effects of therapies, which will be created in our molecular biology lab.

The engineering and computational branch of the OBi Group strives to harness new technologies and machine learning to design new diagnostic devices and equipment for evaluating and supporting visual processing in the diseased retina. Creating models of neuronal networks of visual pathways to investigate mutual functional relationships among different neuronal cell populations enables us to predict functional changes when certain neurons become disabled under pathological conditions.

OBi Address: Kasprzaka 44/52, 01-224 Warsaw
Phone number: +48 22 343 32 04
Group Leader: afoik@ichf.edu.pl
Team Coordinator: aposluszny@ichf.edu.pl

Latest publications:

F. Gao, E. Tom, C. Rydz, W. Cho, A. V. Kolesnikov, Y. Sha, A. Papadam, S. Jafari, A. Joseph, A. Ahanchi, N. Balalaei Someh Saraei, D. Lyon, A. Foik, Q. Nie, F. Grassmann, V. J. Kefalov, D. Skowronska-Krawczyk “Polyunsaturated Fatty Acid - mediated Cellular Rejuvenation for Reversing Age-related Vision Decline” bioRxiv., 2024.07.01.601592. (2024) https://www.biorxiv.org/content/10.1101/2024.07.01.601592v1

H. Leinonen, J. Zhang, L. M. Occelli, U. Seemab, E. H. Choi, L. Felipe L P Marinho, J. Querubin, A. V. Kolesnikov, A. Galinska, K. Kordecka, T. Hoang, D. Lewandowski, T. T. Lee, E. E. Einstein, D. E. Einstein, Z. Dong, P. D. Kiser, S. Blackshaw, V. J. Kefalov, M. Tabaka, A. Foik, S. M. Petersen-Jones, K. Palczewski “A combination treatment based on drug repurposing demonstrates mutation-agnostic efficacy in pre-clinical retinopathy models” Nat Commun., 15(1):5943. (2024) https://www.nature.com/articles/s41467-024-50033-5

R. Hołubowicz, S. W. Du, J. Felgner, R. Smidak, E. H Choi, G. Palczewska, C. Rodrigues Menezes, Z. Dong, F. Gao , O. Medani, A. L. Yan, M. W. Hołubowicz, P. Z. Chen, M. Bassetto, E. Risaliti, D. Salom , J. N. Workman, P. D. Kiser, A. T. Foik, D. C. Lyon, G. A. Newby, D. R. Liu, P. L Felgner, K. Palczewski “Safer and efficient base editing and prime editing via ribonucleoproteins delivered through optimized lipid-nanoparticle formulations” Nat Biomed Eng. (2024) https://www.nature.com/articles/s41551-024-01296-2

P. Węgrzyn, W. Kulesza, M. Wielgo, S. Tomczewski, A. Galińska, B. Bałamut, K. Kordecka, O. Cetinkaya, A. Foik, R. J. Zawadzki, D. Borycki, M. Wojtkowski, A. Curatolo „In vivo volumetric analysis of retinal vascular hemodynamics in mice with spatio-temporal optical coherence tomography” Neurophotonics. 11(4):0450031-4500322. (2024) https//doi: 10.1117/1.NPh.11.4.045003

Z. J. Engfer, D. Lewandowski, Z. Dong, G. Palczewska, J. Zhang, K. Kordecka, J. Płaczkiewicz, D. Panas, A. T. Foik, M. Tabaka, and Krzysztof Palczewski „Distinct mouse models of Stargardt disease display differences in pharmacological targeting of ceramides and inflammatory responses” Proc Natl Acad Sci U S A, 120(50):e2314698120., (2023) https://www.pnas.org/doi/10.1073/pnas.2314698120