OPUS 27 / NSC

Project title: Characterization of sensitivity of cones and rods to two-photon stimulation

Project leader: dr Katarzyna Komar

Two-photon stimulation of photoreceptors will be studied using dedicated optical systems and measurement techniques based on visual psychophysics (flicker photometry), optoretinography, and electrophysiology. With the flicker photometry, the spectral two-photon sensitivity of individual photoreceptor types will be measured. We will compare the results with those obtained by optoretinography, which is based on optical tomography OCT and allows us to assess the extent to which photoreceptors are stimulated by light by analyzing changes occurring in the image of the retinal layers. We will also study visual evoked potentials and changes in the action potentials of retinal neurons induced by two-photon stimuli.

The project results will expand the knowledge of human visual perception, provide a better understanding of the mechanisms by which pulsed laser beams interact with the living eye, and allow us to collect the data necessary for further applications of the two-photon vision phenomenon.

The total funding of the project is 1 998 604 PLN.